ABSTRACT
Objective To investigate whether NO can relieve hypertensive cerebrovascular and renal injury by regulating the ratio of peripheral blood T lymphocyte subsets and reducing proinflammatory cytokine release . Methods Twelve-week SHR and WKY rats were randomly divided into three groups :WKY group ,SHR group and SHR+NO intervention group ,with 6 rats in each group .Rats in SHR+ NO intervention group were injected intraperitoneally with sodium nitroprusside according to 10 μg/(kg · d) for 4 weeks ,while WKY group and SHR group were injected intraperitoneally with an equal amount of saline for 4 weeks .After measuring the tail artery blood pressure ,basilar artery and renal pathological changes were observed by HE staining ;the rates of CD4+ /CD8+ and CD4+ CD25+ T cells were detected by flow cytometry ;and the expression of TNF-α was detected by ELISA .Results The expressions of CD4+ /CD8+ and TNF-αin SHR group were significantly higher than those in WKY group (P<0 .01) while the rate of CD4+ CD25+ in SHR group was significantly lower than that in WKY group (P<0 .01) .The expressions of CD4+ /CD8+ and TNF-α in SHR+ NO intervention group were significantly lower than those in SHR group whereas the rate of CD4+ CD25+ was significantly higher than that in SHR group (P<0 .05) .Conclusion NO can improve the target organ damage caused by hypertension by regulating the peripheral blood T lymphocyte subsets ratio and reducing the release of proinflammatory factors .
ABSTRACT
Objective To investigate the expression of transmembrane protein 16A (TMEM16A)in the cochlea of guinea pigs and its relationship with the age-related hearing loss.Methods We used auditory brainstem response (ABR)to explore the changes of hearing in guinea pigs of different age (groups of 2 w,3 m,1 y,and D-galactose).The distribution and expression of TMEM16A in the cochlea were detected by immunofluorescence and Western blot.Results ABR threshold was gradually increased,with significant difference between D-gal and the other three groups (P <0.01).TMEM16A was expressed in the cochlear striae vascularis at different ages,and the expression increased with age before 1 y (P <0.05). However, its level was increased in D-gal group and significantly differed from that in 3 m and 1 y groups (P < 0.05 ).Conclusion The change in TMEM16A expression in the cochlear striae vascularis of guinea pigs may be related to age-related hearing loss.
ABSTRACT
Objective To investigate the expression of transmembrane protein 16A (TMEM16A)in the cochlea of guinea pigs and its relationship with the age-related hearing loss.Methods We used auditory brainstem response (ABR)to explore the changes of hearing in guinea pigs of different age (groups of 2 w,3 m,1 y,and D-galactose).The distribution and expression of TMEM16A in the cochlea were detected by immunofluorescence and Western blot.Results ABR threshold was gradually increased,with significant difference between D-gal and the other three groups (P <0.01).TMEM16A was expressed in the cochlear striae vascularis at different ages,and the expression increased with age before 1 y (P <0.05). However, its level was increased in D-gal group and significantly differed from that in 3 m and 1 y groups (P < 0.05 ).Conclusion The change in TMEM16A expression in the cochlear striae vascularis of guinea pigs may be related to age-related hearing loss.